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Establishment of a Screening System for Selection of siRNA Target Sites Directed against Hepatitis B Virus Surface GeneXiu-Min ZHOU1,3, Ju-Sheng LIN1, Yi SHI3, De-An TIAN2, Huan-Jun HUANG2, He-Jun ZHOU1, and You-Xin JIN3*1Institute of Liver Diseases, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 3State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, ChinaAbstract To study the inhibitory effects of plasmid-derived small interfering RNA (siRNA) and synthetic siRNA on the expression of the hepatitis B virus surface (HBs) gene, three plasmid-derived siRNAs and one synthetic siRNA that complement the coding region of the HBs gene were The HBs expression plasmid pHBs-EGFP was also HeLa cells were co-transfected with pHBs-EGFP and the above siRNA The HBs mRNA quantities were measured by reverse-transcription PCR, and the level of HBs-EGFP fusion protein was quantified by fluorescent The concentrations of the hepatitis B virus surface antigen (HBsAg) derived from the culture supernatant of transfected HepG15 cells were measured by an enzyme-linked immunosorbent assay (ELISA) The results showed that the three plasmid-derived siRNAs and the synthetic siRNA can effectively reduce the quantities of HBs mRNA and The plasmid-derived siRNA psiRNA1 was found to be the most effective inhibitor of HBs It can inhibit HBs-EGFP expression by 3% and suppress HBs mRNA by 6% To further substantiate the above observations, psiRNA1 was transfected into HepG15 cells (an HBV secreting cell line) The transfections resulted in almost complete blockage of HBsAg production, whereas control vector-transfected cells secreted high levels of HBsAg 7 days post- In conclusion, our data suggests that RNA interference (RNAi) is an efficient approach for reducing the level of HBs transcripts and proteins and for suppressing HBsAg Key words RNA interference (RNAi); small interference RNA (siRNA); hepatitis B virus surface gene (HBs gene)
300 评论

佳佳13817062298

是全球最权威的杂志。 如果专说面向物理学科的杂志,主要有: Review of Modern Physics (现代物理评论)。影响因子在20多 Physics Reports (物理报道)影响因子20多。 以上两部杂志 都是约稿性质的杂志。专门向世界级物理学家约稿。不接受自由投稿。 接下来:Physical Review Letters 物理评论快报 影响因子 7~8 Physical Review 物理评论 (分 A B C D E 几个系列,平均影响因子3~4) Physics Letters 物理快报 (分 A 和 B, 平均影响因子 4左右) Applied Physics Letters 应用物理快报 影响因子 4 以上再接下来European Physical Journal 欧洲物理杂志 Journal of Physics 物理杂志 (可能就是你说的物理学杂志)…………还有一些物理一级学科下的二级学科杂志也很名气,但太多了。

264 评论

小小小花花儿

1 Journal of personality and social psychology 人格与社会心理学杂志 美Journal of Personality and Social Psychology publishes papers in all areas of personality and social psychology and emphasizes empirical reports, but may include specialized theoretical, methodological, and review The journal is divided into three independently edited Attitudes and Social Cognition addresses those domains of social behavior in which cognition plays a major role, including the interface of cognition with overt behavior, affect, and Among topics covered are the formation, change, and utilization of attitudes, attributions, and stereotypes, person memory, self-regulation, and the origins and consequences of moods and emotions insofar as these interact with Of interest also is the influence of cognition and its various interfaces on significant social phenomena such as persuasion, communication, prejudice, social development, and cultural Interpersonal Relations and Group Processes focuses on psychological and structural features of interaction in dyads and Appropriate to this section are papers on the nature and dynamics of interactions and social relationships, including interpersonal attraction, communication, emotion, and relationship development, and on group and organizational processes such as social influence, group decision making and task performance, intergroup relations and aggression, prosocial behavior and other types of social Personality Processes and Individual Differences publishes research on all aspects of personality It includes studies of individual differences and basic processes in behavior, emotions, coping, health, motivation, and other phenomena that reflect Articles in areas such as personality structure, personality development, and personality assessment are also appropriate to this section of the journal, as are studies of the interplay of culture and personality and manifestations of personality in everyday 2 Psychological bulletin 心理学公报 美《心理学公报》(Psychological bulletin双月刊)由美国心理学会(American Psychological Association)主办并出版,是享誉国际的心理学领域顶级期刊。研究及评论心理学的最新发展情况。所载论文既反映了心理学研究领域的最新发展,同时也成为沟通心理学所属各研究领域、心理学与相关学科关系的桥梁。研究内容涉及实验心理学的社会影响、心理学的目标建设,大脑不对称的视觉空间频率模式、精神分裂症的社会认知、宗教和心理治疗过程及心理治疗成果方面的实验研究。Psychological Bulletin publishes evaluative and integrative research reviews and interpretations of issues in scientific Primary research is reported only for illustrative Integrative reviews or research syntheses focus on empirical studies and seek to summarize past research by drawing overall conclusions from many separate investigations that address related or identical A research synthesis typically presents the authors' assessments ofthe state of knowledge concerning the relations of interest;critical assessments of the strengths and weaknesses in past research; andimportant issues that research has left unresolved, thereby directing future research so it can yield a maximum amount of new Both cumulative and historical approaches (, ones that organize a research literature by highlighting temporally unfolding developments in a field) can be Integrative research reviews that develop connections between areas of research are particularly Manuscripts dealing with topics at the interface of psychological sciences and society are welcome, as are evaluations of applied psychological therapies, programs, and Expository articles may be published if they are deemed accurate, broad, clear, and Methodological articles that previously were submitted to Psychological Bulletin should now be submitted toPsychological M Original theoretical articles should be submitted to Psychological Review, even when they include summaries of Research syntheses should be submitted to Psychological Bulletin even when they develop integrated theoretical 3 Psychological review 心理学评论 美《心理学评论》(Psychological Review季刊)由美国心理学会(American Psychological Association)主办并出版,是心理学最富盛名的期刊之一。旨在对科学心理学进行理论上的探讨和研究。刊载在心理学各个领域取得重大理论成就的研究性文章,也涉及某一特定领域的非主流理论的系统性评价。 ISSN: 0033-295X,季刊,1894年创刊;2008年影响因子为765 期刊网址: Review publishes articles that make important theoretical contributions to any area of scientific psychology, including systematic evaluation of alternative Papers mainly focused on surveys of the literature, problems of method and design, or reports of empirical findings are not There is no upper bound on the length of Psychological Review However, authors who submit papers with texts longer than 25,000 words will be asked to justify the need for their Psychological Review also publishes, as Theoretical Notes, commentary that contributes to progress in a given subfield of scientific Such notes include, but are not limited to, discussions of previously published articles, comments that apply to a class of theoretical models in a given domain, critiques and discussions of alternative theoretical approaches, and meta-theoretical commentary on theory testing and related 4 Behavioral & brain sciences 行为和脑科学杂志 英BBS is the internationally renowned journal with the innovative format known as Open Peer C Particularly significant and controversial pieces of work are published from researchers in any area of psychology, neuroscience, behavioural biology or cognitive science, together with 10-25 commentaries on each article from specialists within and across these disciplines, plus the author's response to The result is a fascinating and unique forum for the communication, criticism, stimulation, and particularly the unification of research in behavioural and brain sciences from molecular neurobiology to artificial intelligence and the philosophy of the 5 Psychological reports 心理学报告 美 6 Journal of consulting and clinical psychology咨询心理学与临床心理学杂志7 Perceptual motor skills 感知与运动技能 英8 Psychological medicine 心理医学 美9 Journal of cognitive neuroscience 认知神经科学杂志 美10 Journal of experimental Learning,memory,and cognition 实验心理学杂志学习、记忆和认识 美11 Journal of abnormal psychology 变态心理学杂志 美12 Developmental psychology 发展心理学 美13 Psychosomatic medicine 身心医学 美14 Journal of experimental Human perception and performance实验心理学杂志人类知觉与行为 美15 Personality and individual differences 个性与个体差异 英16 Psychophysiology 心理生理学 英17 Annual review of psychology 心理学年度评论 美18 Behaviour research and therapy 行为研究和治疗 英19 Pharmacology, biochemistry and behavior 药理学、生物化学和行为 美20 Journal of applied psychology 应用心理学杂志 美21 Journal of memory and language记忆与语言杂志 美22 The Journal of child psychology and psychiatry & allied disciplines儿童心理学、精神病学及相关学科杂志 英23 Journal of affective disorders 情感紊乱杂志 荷兰24 Cognitive psychology 认知心理学 美25 Psychological science 心理科学 美26 Cognition 认知 荷兰27 Health psychology 健康心理学 美28 Brain research 大脑研究 荷兰29 Perception & psychophysics知觉与心理学 美30 Personality & social psychology bulletin 个性与社会心理学公报 美31 Vision research视觉研究 英32 Psychopharmacology bulletin 精神鞠理学公报 美33 Journal of studies on alcohol酒精研究杂志 美34 Psychology and aging心理学与衰老 美35 Brain and language 大脑与语言 美36 Memory and cognition 记忆与认知 美37 Journal of psychosomatic research身心研究杂志 美38 Journal of experimental General 实验心理学杂志总论 美39 International journal of eating disorders 国际进食障碍杂志 美40 Journal of educational psychology 教育心理学杂志 美41 American psychologist 美国心理学家 美42 Professional psychology, research and practice专业专理学,研究及实践 美43 Child abuse & neglect 虐待与忽视儿童研究 英44 Neuropsychology 神经心理学 美45 Organizational behavior and human decision processes组织行为与人类决策过程 美46 Behavioural brain research 行为大脑研究 荷兰47 Journal of applied behavior analysis应用行为分析杂志 美48 Perception 知觉 英49 Behavior therapy 行为治疗 美50 Psychological assessment 心理评价 美51 Journal of abnormal child psychology 变态儿童心理学杂志 美52 Journal of counseling psychology咨询心理学杂志 美53 Journal of personality assessment个性评估杂志 美54 Journal of traumatic stress 创伤应激反应杂志 美55 Cognitive neuropsychology 认知神经心理学 英56 Ethology 行为学 德57 Ergonomics 人机学 英58 Journal of experimental child psychology 实验儿童心理学杂志 美59 Law and human behavior 法律和人类行为 美60 Sex roles性别作用 美61 Journal of vocational behavior职业行为杂志 美62 Journal of personality 个性杂志 美63 Addictive behaviors成瘾行为 英64 Journal of experimental social psychology实验社会心理学杂志 美65 Journal of comparative psychology 比较心理学杂志 美66 The International journal of psycho-analysis 国际心理分析杂志 英67 Cognitive therapy and research 认知治疗与研究 美68 Clinical psychology review 临床心理学评论 英69 Journal of sport & exercise psychology运动与训练心理学杂志 美70 Educational and psychological measurement 教育与心理测量 英71 British journal of clinical psychology英国临床心理学杂志 英72Journal of experimental psycholoy Animal behavior processes实验心理学杂志动物行为过程 美 73 British journal of psychology 英国心理学杂志 英74 Behavior research methods,instruments & computers 行为研究方法、仪器与计算机 英75 Social psychology quarterly 社会心理学季刊 美76 European journal of social psychology欧洲社会心理学杂志 英77 Infant behavior & development 婴儿行为与发育 美78 Neuropsychologia神经心理学 英79 Journal of pediatric psychology 儿科心理学杂志 英80 Behavioral neuroscience 行为神经科学 美81 The Journal of social psychology 社会心理学杂志 美82 Cognitive science 认知科学 美83 Journal of clinical child psychology临床儿童心理学杂志 美84 Biological psychology 生物心理学 荷兰85 Psychobiology 心理生物学 美86 American journal of psychology 美国心理学杂志 美87 Brain and cognition 大脑与认知 美88 Language and cognitive processes 语言与认知过程 英89 Memory 记忆 英90 Scandinavian journal of psychology 斯堪的纳维亚心理学杂志 英

172 评论

陆老头11

OncoTargets and Therapy 创刊于2008年,是一本非常年轻的期刊,由出版商Dove 发布,主编是美国堪萨斯大学医学院的副教授 Faris Farassati 博士,研究领域分别为Biotechnology & Applied Microbiology 和 Oncology,目前分别在同领域中排名56/156 和 119/244位,JCR Q2,中科院3区,2019年影响因子 1)影响因子:OncoTargets and Therapy 杂志的影响因子近年来一直在稳定增长,2015-2019年的SCI影响因子分别为272、612、656、046、337,增长趋势稳定。 2)自引率: OncoTargets and Therapy 在近几年的自引率,2019年1%,这个比例还是比较低的。 3)审稿周期:从提交到第一个决定需要22天,这个速度是比较快的。同时,官网给出的接收率为24%! 4)版面费:根据作者所在位置的不同,收取不同的费用,中国是2500美元,约合人民币17000,彩色插图不收取额外费用。 回答参考资料

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